Alexa Fluor(R) 594 Streptavidin from BioLegend

Product Specs
ItemAlexa Fluor(R) 594 Streptavidin
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number405240
Quantity100 µg
ApplicationsImmunocytochemistry (ICC), Immunohistochemistry (IHC)-F, Immunohistochemistry (IHC)-P
Concentration0.50 mg/mL
Conjugate/Tag/LabelAlexa Fluor® 594
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
SpeciesHuman, Mouse, Rat, All Species
TargetBiotin
TypeStreptavidin

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A modified SELEX approach to identify DNA aptamers with binding specificity to the major histocompatibility complex presenting ovalbumin model antigen†RSC AdvancesOctober 31, 2023Yang Lin, Cho-Yi Chen, Yu-Chia Ku, Li-Chin Wang, Chia-Chien Hung, Zhi-Qian Lin, Bing-Hong Chen, Jui-Tse Hung, Yi-Chen Sun, Kai-Feng Hung30 minutes followed by staining with the 1 : 500 secondary antibody Streptavidin Alexa-594 (Cat. no.405240, BioLegend, USA) at 4 °C for 30 minutes. Nuclei were stained with 300 nM DAPI at 4 °C for 15 minutes.

(2) T Cells Expressing a Modified FcγRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid TumorsCancer immunology researchJune 2, 2023Rasoulouniriana D, Santana-Magal N, Gutwillig A, Farhat-Younis L, Tal L, Amar S, Milyavsky M, Muddineni SSNA, Solomon N, Shpilt H, et al.Human HER2/ErbB2 Protein (ECD) Biotinylated (Sino Biological, #10004-HCCH-B) and Alexa Fluor 594 Streptavidin (BioLegend, #405240). Images were collected using a Zeiss LSM800 confocal laser scanning microscope using Objective Plan-Apochromat

(3) Altered Tau Kinase Activity in rTg4510 Mice after a Single Interfaced CHIMERA Traumatic Brain InjuryInternational Journal of Molecular SciencesMay 29, 2023Wai Hang Cheng, Honor Cheung, Amy Kang, Jianjia Fan, Jennifer Cooper, Mehwish Anwer, Carlos Barron, Anna Wilkinson, Grace Hu, Jefferey Yue, et al.blocked, and probed with p62 antibody (NEB D5L7G, 1:800, Ipswich, MA, USA) and streptavidin-594 (Biolegend 405240, 1:500, San Diego, CA, USA), using the Mouse on Mouse kit (Vector BMK2202, Newark, USA) per the manufacturer’s

(4) Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cellsbioRxiv : the preprint server for biologyFebruary 13, 2023Parween F, Singh SP, Zhang HH, Kathuria N, Otaizo-Carrasquero FA, Shamsaddini A, Gardina PJ, Ganesan S, Kabat J, Lorenzi HA, et al.washing with Vascular Cell Basal Medium, cells were incubated with Alexa Fluor 594 streptavidin (1:500, 405240; BioLegend) for an additional 30 min. Cells were washed and fixed with 4% paraformaldehyde and after

(5) High-affinity, neutralizing antibodies to SARS-CoV-2 can be made without T follicular helper cellsScience ImmunologyFebruary 4, 2022Jennifer S. Chen et al.BioLegend, 144606) overnight at 4°C. Secondary staining was performed with AF594 streptavidin (BioLegend, 405240) for 1 hr at room temperature. Images were acquired on a Nikon TiE Spinning Disk Confocal Microscope

(6) Improvement of HSV-1 based amplicon vectors for a safe and long-lasting gene therapy in non-replicating cellsMolecular Therapy - Methods & Clinical DevelopmentJune 11, 2021Marie Soukupová et al.biotin anti-mouse/human CD45R/B220 antibody (1:100; 103204, BioLegend) associated with Alexa Fluor 594 Streptavidin (1:250; 405240, BioLegend). All secondary antibodies were incubated at room temperature for 2.5 h. Finally, sections

(7) Multiplexed CRISPR/CAS9-mediated engineering of pre-clinical mouse models bearing native human B cell receptorsThe EMBO journalJanuary 15, 2021Wang X, Ray R, Kratochvil S, Melzi E, Lin YC, Giguere S, Xu L, Warner J, Cheon D, Liguori A, et al.405235 Alexa Fluor 647 Streptavidin,BioLegend,Cat#: 405237 Alexa Fluor 594 Streptavidin,BioLegend,Cat#: 405240 "Pan B‐Cell Isolation Kit II, mouse",Miltenyi Biotec,Cat#: 130‐104‐443 SuperScript™ III Reverse Transcriptase,Thermo Fisher

(8) MEF2C regulates osteoclastogenesis and pathologic bone resorption via c-FOSBone researchJanuary 11, 2021Fujii T, Murata K, Mun SH, Bae S, Lee YJ, Pannellini T, Kang K, Oliver D, Park-Min KH, Ivashkiv LB.goat anti-rabbit IgG antibody (Vector Laboratories #BA-1000), Alexa Fluor 594 Streptavidin (Biolegend #405240), and Fluoroshield Mouting Medium (Abcam #ab104139) were used. Human osteoclast differentiation “Peripheral blood mononuclear cells

(9) A Peptidyl Inhibitor that Blocks Calcineurin–NFAT Interaction and Prevents Acute Lung InjuryJournal of Medicinal ChemistryNovember 12, 2020Patrick G. Dougherty et al.suppliers: α-mouse CD45-PerCP/Cy5.5 and streptavidin-Alexa Fluor 594 from Biolegend (Cat. No. NC0217834 and 405240), α-rabbit IgG-Alexa Fluor 488 from Cell Signaling (Cat. No. 4412S), and Human/Mouse/Rat NFATc3 from

(10) RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic SignaturesImmunoHorizonsNovember 10, 2020Li D, Bradley T, Cain DW, Pedroza-Pacheco I, Aggelakopoulou M, Parks R, Barr M, Xia SM, Scearce R, Bowman C, et al.anti–IL-5 (clone no. TRFK4, catalog no. 504402, 1:100 dilution; BD), and streptavidin-AF594 (catalog no. 405240, 1:3000 dilution; BioLegend) Abs in permeabilization buffer (catalog no. 88-8824; Invitrogen). LIVE/DEAD

(11) A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cellsScientific reportsApril 22, 2020Thuault S, Mamelonet C, Salameh J, Ostacolo K, Chanez B, Salaün D, Baudelet E, Audebert S, Camoin L, Badache A.were from Sigma Life Science. Biotinylated proteins were detected either by Alexa Fluor594 Streptavidin (405240, BioLegend) or Streptavidin-HRP (21130, Thermo Scientific). Proximity biotinylation. Cells stably expressing

(12) Expanding the Dynamic Range of Fluorescence Assays through Single-Molecule Counting and Intensity CalibrationJournal of the American Chemical SocietyOctober 24, 2018Lucas Smith, Manish Kohli, Andrew M. Smith(azide-PEG5000-NHS, 5000 Da) were purchased from Nanocs, Inc. Avidin-labeled phycoerythrin (405203) and Alexa 594 (405240) were purchased from BioLegend. Avidin-labeled Quantum Dot 605 nm (Q10103MP) and FluoSpheres (F8770)

(13) The Chemokine Receptor CCR8 Promotes the Migration of Dendritic Cells into the Lymph Node Parenchyma to Initiate the Allergic Immune ResponseImmunitySeptember 18, 2018Sokol CL, Camire RB, Jones MC, Luster AD.Reagent/Material,Source,Identifier Antibodies:,, Alexa Fluora 594 Streptavidin,Biolegend,Cat #: 405240 Alexa Fluroa 647 Streptavidin,Biolegend,Cat #: 405237 "Anti-Mouse CD8α, Clone: 53-6.7",Biolegend,Cat

(14) IFNAR1-Signalling Obstructs ICOS-mediated Humoral Immunity during Non-lethal Blood-Stage Plasmodium InfectionPLoS pathogensNovember 1, 2016Sebina I, James KR, Soon MS, Fogg LG, Best SE, Labastida Rivera F, Montes de Oca M, Amante FH, Thomas BS, Beattie L, et al.

(15) Engineered Lactobacillus rhamnosus GG expressing IgG-binding domains of protein G: Capture of hyperimmune bovine colostrum antibodies and protection against diarrhea in a mouse pup rotavirus infection modelVaccineJanuary 16, 2014Günaydın G, Zhang R, Hammarström L, Marcotte H.

(16) Essential role for Toll-like receptor 7 (TLR7)-unique cysteines in an intramolecular disulfide bond, proteolytic cleavage and RNA sensingInternational immunologyJuly 1, 2013Kanno A, Yamamoto C, Onji M, Fukui R, Saitoh S, Motoi Y, Shibata T, Matsumoto F, Muta T, Miyake K.

(17) Clinical-grade multipotent adult progenitor cells durably control pathogenic T cell responses in human models of transplantation and autoimmunityJournal of immunology (Baltimore, Md. : 1950)May 1, 2013Reading JL, Yang JH, Sabbah S, Skowera A, Knight RR, Pinxteren J, Vaes B, Allsopp T, Ting AE, Busch S, et al.

(18) IL7Rα contributes to experimental autoimmune encephalomyelitis through altered T cell responses and nonhematopoietic cell lineagesJournal of immunology (Baltimore, Md. : 1950)May 1, 2013Ashbaugh JJ, Brambilla R, Karmally SA, Cabello C, Malek TR, Bethea JR.

(19) Induction of BAFF expression by IFN-γ via JAK/STAT signaling pathways in human intestinal epithelial cellsJournal of leukocyte biologyMarch 1, 2013Woo SJ, Im J, Jeon JH, Kang SS, Lee MH, Yun CH, Moon EY, Song MK, Kim HH, Han SH.

(20) Immune complex-induced inhibition of osteoclastogenesis is mediated via activating but not inhibitory Fcγ receptors on myeloid precursor cellsAnnals of the rheumatic diseasesFebruary 1, 2013Grevers LC, de Vries TJ, Everts V, Verbeek JS, van den Berg WB, van Lent PL.

Write a Review

(1)

11/19/2021Great Streptavidin Stain Matt ArvedsonStreptavidin conjugates are used to detect biotin-stained markers. We use it to detect CCR7 biotin-stained samples.

Alexa Fluor(R) 594 Streptavidin from BioLegend

Description

BioLegend

Citations (27)

Reviews

Alexa Fluor(R) 594 Streptavidin from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map